{"id":647,"date":"2026-03-26T11:15:54","date_gmt":"2026-03-26T14:15:54","guid":{"rendered":"https:\/\/uenf.br\/cbb\/lbct\/?page_id=647"},"modified":"2026-03-30T11:57:11","modified_gmt":"2026-03-30T14:57:11","slug":"plataforma-de-sequenciamento-de-dna-uea-lbct","status":"publish","type":"page","link":"https:\/\/uenf.br\/cbb\/lbct\/plataforma-de-sequenciamento-de-dna-uea-lbct\/","title":{"rendered":"Plataforma de Sequenciamento de DNA &#8211; UEA\/LBCT"},"content":{"rendered":"<p>Sequenciamento de DNA pelo m\u00e9todo de Sanger, utilizando o reagente BigDye Terminator V3.1 e o Analisador Gen\u00e9tico 3500 (Termo Fisher Scientific). As etapas do processo podem ser resumidas abaixo:<\/p>\n<ul>\n<li>Purifica\u00e7\u00e3o dos produtos de PCR (amplicons) com ExoSAP-IT (Thermo Fisher Scientific) ou m\u00e9todo dispon\u00edvel (Plasm\u00eddeos n\u00e3o requerem esta etapa).<\/li>\n<li>Rea\u00e7\u00e3o de Sequenciamento com BigDye Terminator v3.1 (Thermo Fisher Scientific)<\/li>\n<li>Purifica\u00e7\u00e3o dos produtos da rea\u00e7\u00e3o de sequenciamento por precipita\u00e7\u00e3o alco\u00f3lica ou m\u00e9todo dispon\u00edvel.<\/li>\n<li>An\u00e1lise da rea\u00e7\u00e3o de sequenciamento por eletroforese capilar no Analisador Gen\u00e9tico ABI 3500 (Thermo Fisher Scientific).<\/li>\n<li>Revis\u00e3o dos dados gerados e envio aos pesquisadores respons\u00e1veis.<\/li>\n<\/ul>\n<p>Respons\u00e1vel: Prof. Glauber Monteiro Dias<\/p>\n<p><strong>Formul\u00e1rio para cota\u00e7\u00e3o e sequenciamento:<\/strong><\/p>\n<p><a href=\"https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLSdlbl5rDvR_x_Qw3Sm_MbV_FsDxFVXNrogX62v4oBJC4csV8A\/viewform?usp=header\">https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLSdlbl5rDvR_x_Qw3Sm_MbV_FsDxFVXNrogX62v4oBJC4csV8A\/viewform?usp=header<\/a><\/p>\n<div id=\"attachment_656\" style=\"width: 1034px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-656\" class=\"wp-image-656 size-large\" src=\"https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-1024x461.jpg\" alt=\"\" width=\"1024\" height=\"461\" srcset=\"https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-1024x461.jpg 1024w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-300x135.jpg 300w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-768x346.jpg 768w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-1536x691.jpg 1536w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-2048x922.jpg 2048w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-1200x540.jpg 1200w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/20220825_154205-320x144.jpg 320w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><p id=\"caption-attachment-656\" class=\"wp-caption-text\">Analisador Gen\u00e9tico ABI 3500 do LBCT\/UEA<\/p><\/div>\n<div id=\"attachment_660\" style=\"width: 1034px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-660\" class=\"size-large wp-image-660\" src=\"https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-1024x328.png\" alt=\"\" width=\"1024\" height=\"328\" srcset=\"https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-1024x328.png 1024w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-300x96.png 300w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-768x246.png 768w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-1536x492.png 1536w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-2048x656.png 2048w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-1200x384.png 1200w, https:\/\/uenf.br\/cbb\/lbct\/files\/2026\/03\/eletroferograma-320x103.png 320w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><p id=\"caption-attachment-660\" class=\"wp-caption-text\">Eletroferograma de sequ\u00eancia de DNA<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Sequenciamento de DNA pelo m\u00e9todo de Sanger, utilizando o reagente BigDye Terminator V3.1 e o Analisador Gen\u00e9tico 3500 (Termo Fisher&#8230;<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-647","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/uenf.br\/cbb\/lbct\/wp-json\/wp\/v2\/pages\/647","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/uenf.br\/cbb\/lbct\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/uenf.br\/cbb\/lbct\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/uenf.br\/cbb\/lbct\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/uenf.br\/cbb\/lbct\/wp-json\/wp\/v2\/comments?post=647"}],"version-history":[{"count":0,"href":"https:\/\/uenf.br\/cbb\/lbct\/wp-json\/wp\/v2\/pages\/647\/revisions"}],"wp:attachment":[{"href":"https:\/\/uenf.br\/cbb\/lbct\/wp-json\/wp\/v2\/media?parent=647"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}